Therefore, although attempts have been reported to separately detect SCCA1 and SCCA2, as these two isoforms have distinct biological functions [8], [9], we choose to use Clone FL-390 for the subsequent immunoblotting and IHC assays, because 1) Clone FL-390 offers better efficiency for both immunoblotting and IHC analysis; and 2) based on current medical studies, an assay realizing both SCCA1 and SCCA2 is recommended for ideal medical level of sensitivity [10]

Therefore, although attempts have been reported to separately detect SCCA1 and SCCA2, as these two isoforms have distinct biological functions…