Notably, pellets from the initial centrifugation steps (200?g, 1,500?g, and 16,000?g, expected to contain the nuclei and large intracellular debris (200?g), as well as highly crosslinked and stable fractions of cytoskeleton (1,500?g and 16,000?g)) contained proportionally larger fraction of R-actin compared to the -actin content in the same fractions (2C5 fold enrichment)
Notably, pellets from the initial centrifugation steps (200?g, 1,500?g, and 16,000?g, expected to contain the nuclei and large intracellular debris…